Cell-Free Protein Expression
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Wieslaw Kudlicki
List price: $149.00 |
About this bookFollowing its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerical competition have driven the development of a variety of systems with increased productivity, improved protein quality and relatively low production costs. As a result, technology has generated myriad applications that have enabled advances in fields as diverse as systems biology, structural biology, and drug discovery. Cell-Free Protein Expression describes and expands upon many of these applications. The volume has been divided into six main sections. In the first section, many of the most popular sources of cell-free lysates are introduced. The second section focuses on extraordinary advances made in the Escherichia coli-based systems that have enabled reconstitution of the entire translational process, incorporation of post-translational modifications, yield increase, and production of functional membrane proteins. This progress extends the usefulness of cell-free systems into structural biology applications described in the third section and high-content platforms like protein microarrays discussed in the fourth section. The final two sections cover the use of cell-free protein expression technologies in the rational design and directed evolution of proteins within the scientific community. |
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Table of contents1. The Role of Cell-Free Rabbit Reticulocyte Expression Systems 2. Advances in Insect-Based Cell-Free Protein Expression 3. Advantages and Applications of the Batch-Formatted E. coli 4. Energetics in Escherichia coli-Based Batch Cell-Free Systems 5. Disulfide Bond Formation in Bacteria-Based Cell-Free Protein Expression 6. The PURE System: A Minimal Cell-Free Translation System 7. Cell-Free Expression Approaches for the Production and Characterization
8. Cell-Free Expression for Protein NMR 9. Cell-Free Synthesis of Membrane Proteins for X-Ray Crystallography 10. Bacterial Cell-Free Expression Systems for High-Throughput 11. Cell-Free Protein Synthesis for Protein Microarrays 12. Cell-Free Protein Expression Screening and Protein Immobilization Using
13. Cell-Free Protein Expression Labeling with Fluorophores 14. Cell-Free Synthesis of Defined Protein Conjugates by Site-Directed 15. C-Terminal Labeling of Proteins Using Fluorescently Conjugated 16. Translation Engineering and Synthetic Biology 17. Accelerated Protein Evolution Using Ribosome Display 18. Application of in Vitro Virus (IVV) Technique for High-Throughput |
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