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Research Paper

Identification of genome-derived vaccine candidates conserved between human and mouse-adapted strains of H. pylori

Leonard Moise, Julie A. McMurry, Jacques Pappo, Dong-Soo Lee, Steven F. Moss, William D. Martin and Anne S. De Groot

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Computational methods accelerate vaccine development by rapid identification of potential vaccine candidates. We screened the Helicobacter pylori J99 and 26695 genomes for T-cell epitopes using the epitope mapping algorithm EpiMatrix and selected 150 sequences for experimental validation in a pre-clinical mouse model. Because strains of H. pylori that infect humans do not generally infect mice, and the sequence of the mouse-adapted “Sydney” strain (SS1) is not publicly available, we used targeted PCR to confirm that epitopes we computationally predicted from the human H. pylori isolates J99 and 26695 are conserved in SS1. Epitopes conserved in SS1 were further analyzed for binding to MHC in vitro and for antigenicity in infected mice to select candidates for an epitope-based vaccine.

Authors

Leonard Moise

Brown University; Providence, Rhode Island USA

Julie A. McMurry

EpiVax, Inc.; Providence, Rhode Island USA

Jacques Pappo

EpiVax, Inc.; Providence, Rhode Island USA

Dong-Soo Lee

Brown University; Providence, Rhode Island USA

Steven F. Moss

Brown University; Providence, Rhode Island USA

William D. Martin

EpiVax, Inc.; Providence, Rhode Island USA

Anne S. De Groot

Brown University; Providence, Rhode Island USA

Purchase article for $19

Subscribe to this journal for $79/year