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Article Addendum

A vacuolar class III peroxidase and the metabolism of anticancer indole aklaloids in Catharanthus roseus: Can peroxidases, secondary metabolites and arabinogalactan proteins be partners in microcompartmentation of cellular reactions?

Mariana Sottomayor, Patrícia Duarte, Raquel Figueiredo and Alfonso Ros Barceló

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Plants possess a unique metabolic diversity commonly designated as secondary metabolism, of which the anticancer alkaloids from Catharanthus roseus are among the most studied. Recently, in a classical function-to-protein-to-gene approach, we have characterized the main class III peroxidase (Prx) expressed in C. roseus leaves, CrPrx1, implicated in a key biosynthetic step of the anticancer alkaloids. We have shown the vacuolar sorting determination of CrPrx1 using GFP fusions and we have obtained further evidence supporting the role of this enzyme in alkaloid biosynthesis, indicating the potential of CrPrx1 as a molecular tool for the manipulation of alkaloid metabolism. Here, we discuss how plant cells may regulate Prx reactions. In fact, Prxs form a large multigenic family whose members accept a broad range of substrates and, in their two subcellular localizations, the cell wall and the vacuole, Prxs co-locate with a large variety of secondary metabolites which can be accepted as substrates. How then, are Prx reactions regulated? Localization data obtained in our lab suggest that arabinogalactan proteins (AGPs) and Prxs may be associated in membrane microdomains, evocative of lipid rafts. Whether plasma membrane and/or tonoplast microcompartmentation involve AGPs and Prxs and whether this enables metabolic channeling determining Prx substrate selection are challenging questions ahead.

Addendum to: Costa MM, Hilliou F, Duarte P, Pereira LG, Almeida I, Leech M, Memelink J, Barceló AR, Sottomayor M. Molecular cloning and characterization of a vacuolar class III peroxidase involved in the metabolism of anticancer alkaloids in Catharanthus roseus. Plant Physiol 2008; 146:403-17.

Authors

Mariana Sottomayor

IBMC—Instituto de Biologia Molecular e Celular; Departamento de Botânica; Faculdade de Ciências; Universidade do Porto; Porto, Portugal

Patrícia Duarte

IBMC—Instituto de Biologia Molecular e Celular; Universidade do Porto; Porto, Portugal

Raquel Figueiredo

IBMC-Instituto de Biologia Molecular e Celular; Departamento de Botânica; Faculdade de Ciências; Universidade do Porto; Porto, Portugal

Alfonso Ros Barceló

Departmento de Biología Vegetal; Facultad de Biología; Universidad de Murcia; Murcia, Spain

Purchase article for $19

Subscribe to this journal for $79/year