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Article Addendum

Are extracellular matrix surface network components involved in signalling and protective function?

Marzena Popielarska-Konieczna, Małgorzata Kozieradzka-Kiszkurno, Joanna Świerczyńska, Grzegorz Góralski , Halina Ślesak and Jerzy Bohdanowicz

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Endosperm is an interesting model for in vitro experiments, because of its unique origin, development and ploidy level. Here we used Actinidia deliciosa endosperm-derived callus to investigate morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long - term culture. SEM and TEM analysis showed that ECM is a heterogenous layer which consists of amorphous, dark – staining material, osmiophilic granules and reticulated fibres outside the outer callus cell wall. This structure may serve as a structural marker of morphogenic competence in endosperm – derived callus, because of its presence on the surface of callus forming morphogenic domains and its disappearance during organ growth. Based on immunolabelling, histochemistry, solvent and enzyme treatments, we suggest that pectins and lipids are components of the ECM layer. These results might indicate protective, water retention and/or cell communication functions for this ECM layer.

Addendum to: Popielarska-Konieczna M, Kozieradzka-Kiszkurno M, Świerczyńska J, Góralski G, Ślesak H, Bohdanowicz J. Ultrastructure and histochemical analysis of extracellular matrix surface network in kiwifruit endosperm-derived callus culture. Plant Cell Rep 2008; 27:1137-45.

Authors

Marzena Popielarska-Konieczna

Department of Plant Cytology and Embryology, Jagiellonian University, Cracow, Poland

Małgorzata Kozieradzka-Kiszkurno

Department of Plant Cytology and Embryology, University of Gdańsk, Gdańsk, Poland

Joanna Świerczyńska

Department of Plant Cytology and Embryology, University of Gdańsk, Gdańsk, Poland

Grzegorz Góralski

Department of Plant Cytology and Embryology, Jagiellonian University, Cracow, Poland

Halina Ślesak

Department of Plant Cytology and Embryology, Jagiellonian University, Cracow, Poland

Jerzy Bohdanowicz

Department of Plant Cytology and Embryology, University of Gdańsk, Gdańsk, Poland

Purchase article for $19

Subscribe to this journal for $79/year