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Article Addendum

Vacuolar and cytoskeletal dynamics during elicitor-induced programmed cell death in tobacco BY-2 cells

Takumi Higaki, Yasuhiro Kadota, Tatsuaki Goh, Teruyuki Hayashi, Natsumaro Kutsuna, Toshio Sano, Seiichiro Hasezawa and Kazuyuki Kuchitsu

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Responses of plant cells to environmental stresses often involve　morphological changes, differentiation and redistribution of various organelles and cytoskeletal network. Tobacco BY-2 cells provide excellent model system for in vivo imaging of these intracellular events. Treatment of the cell cycle-synchronized BY-2 cells with a proteinaceous oomycete elicitor, cryptogein, induces highly synchronous programmed cell death (PCD) and provide a model system to characterize vacuolar and cytoskeletal dynamics during the PCD. Sequential observation revealed dynamic reorganization of the vacuole and actin microfilaments during the execution of the PCD. We further characterized the effects cryptogein on mitotic microtubule organization in cell cycle-synchronized cells. Cryptogein treatment at S phase inhibited formation of the preprophase band, a cortical microtubule band that predicts the cell division site. Cortical microtubules kept their random orientation till their disruption that gradually occurred during the execution of the PCD twelve hours after the cryptogein treatment. Possible molecular mechanisms and physiological roles of the dynamic behavior of the organelles and cytoskeletal network in the pathogenic signal-induced PCD are discussed.

Addendum to: Higaki T, Goh T, Hayshi T, Kutsuna N, Kadota Y, Hasezawa S, Sano T, Kuchitsu K. Elicitor-induced cytoskeletal rearrangement relates to vacuolar dynamics and execution of cell death: In vivo imaging of hypersensitive cell death in tobacco BY-2 cells. Plant Cell Physiol 2007; 48:1414-25.

Authors

Takumi Higaki

Department of Integrated Biosciences; Graduate School of Frontier Sciences; The University of Tokyo; Kashiwanoha Kashiwa, Chiba, Japan; Department of Applied Biological Science; Tokyo University of Science; Noda, Chiba, Japan

Yasuhiro Kadota

Department of Applied Biological Science; Tokyo University of Science; Noda, Chiba, Japan

Tatsuaki Goh

Department of Applied Biological Science; Tokyo University of Science; Noda, Chiba, Japan

Teruyuki Hayashi

Department of Applied Biological Science; Tokyo University of Science; Noda, Chiba, Japan

Natsumaro Kutsuna

Department of Integrated Biosciences; Graduate School of Frontier Sciences; The University of Tokyo; Kashiwanoha Kashiwa, Chiba, Japan; Institute for Bioinformatics Research and Development (BIRD); Japan Science and Technology Agency (JST)

Toshio Sano

Department of Integrated Biosciences; Graduate School of Frontier Sciences; The University of Tokyo; Kashiwanoha Kashiwa, Chiba, Japan; Institute for Bioinformatics Research and Development (BIRD); Japan Science and Technology Agency (JST)

Seiichiro Hasezawa

Department of Integrated Biosciences; Graduate School of Frontier Sciences; The University of Tokyo; Kashiwanoha Kashiwa, Chiba, Japan; Institute for Bioinformatics Research and Development (BIRD); Japan Science and Technology Agency (JST)

Kazuyuki Kuchitsu

Department of Applied Biological Science; Tokyo University of Science; Noda, Chiba, Japan; Genome & Drug Research Center; Tokyo University of Science; Noda, Chiba, Japan

Purchase article for $19

Subscribe to this journal for $79/year