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Article Addendum
Enigmatic Brefeldin A
Yu Chung Tse, Sheung Kwan Lam and Liwen Jinag
volume 2 | issue 3
may/june 2007Pages: 199 - 201
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
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The fungal macrocyclic lactone brefeldin A (BFA) has been a useful tool in studying protein trafficking in the secretory and endocytic pathways in plant cells. The development of various GFP-tagged organelle markers expressed in transgenic plant cells has allowed dynamic study of organelles in response to BFA in living cells. Several organelles including the endoplasmic reticulum (ER), the Golgi apparatus and endosomal compartment have been shown to have visible morphological changes in response to BFA treatment, resulting in the formation of BFA-induced aggregated compartments or ER-Golgi hybrids in various plant cells. Using transgenic tobacco BY-2 cells expressing membrane-anchored yellow fluorescent protein (YFP) reporters marking Golgi apparatus or prevacuolar compartment (PVC), we have recently demonstrated that Golgi and PVC organelles have different sensitivity to BFA, where BFA at recoverable high concentrations (50 to 100 μg/ml) also induced PVC or multivesicular body (MVB) to form aggregates in plant cells. We have thus extended the BFA action to plant PVCs/MVBs, which will serve as a useful tool for studying PVC-mediated protein sorting and PVC biogenesis.
Authors
Yu Chung Tse
The Chinese University of Hong Kong, Hong Kong, China
Sheung Kwan Lam
The Chinese University of Hong Kong, Hong Kong, China
Liwen Jinag
The Chinese University of Hong Kong, Hong Kong, China
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.