Recommend Fly to your librarian for 2008. Download form here.
Sign up for Table of Contents Alerts!
Important News: Fly has been accepted for indexing at PubMed.
Email this page
Print this page
Research Papers
A Germline Clone Screen for Meiotic Mutants in Drosophila melanogaster
Scott Page, Rachel J. Nielsen, Kathy Teeter, Cathleen M. Lake, SengKai Ong, Kathleen R. Wright, Kristen L. Dean, Daniel Agne, William D. Gilliland and Scott Hawley
volume 1 | issue 3
May/June 2007Pages: 172 - 181
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.
Using a FLP/FRT-based method to create germline clones, we screened Drosophila chromosome arms 2L and 3R for new female meiotic mutants. The screen was designed to recover mutants with severe effects on meiotic exchange and/or segregation. This screen yielded 11 new mutants, including six alleles of previously known meiotic genes (c(2)M and ald/mps1). The remaining five mutants appear to define at least four new genes whose ablation results in severe meiotic defects. Three of the novel meiotic mutants were identified at the molecular level. Two of these, mcm5A7 and tremF9, define roles in meiotic recombination, while a third, conaA12, is important for synaptonemal complex assembly. Surprisingly, five of the nine mutants for which the lesion has been identified at the molecular level are not the result of mutations characteristic of EMS mutagenesis, but rather due to the insertion of the transposable element Doc. This study demonstrates the utility of germline clone-based screens for the discovery of strong meiotic mutants, including mutations in essential genes, and the use of molecular genetic techniques to map the loci.
Authors
Scott Page
James Cook University; Townsville, Queensland, Australia
Rachel J. Nielsen
Stowers Institute; Kansas City, MO
Kathy Teeter
Stowers Institute; Kansas City, MO
Cathleen M. Lake
Stowers Institute; Kansas City, MO
SengKai Ong
University of Missouri; Kansas City, MO
Kathleen R. Wright
University of Kansas; Lawrence, KS
Kristen L. Dean
University of Kansas; Lawrence, KS
Daniel Agne
University of Kansas; Lawrence, KS
William D. Gilliland
Stowers Institute; Kansas City, MO
Scott Hawley
Stowers Institute; Kansas City, MO
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.