CJC is published 12 times a year.
Sign up for Table of Contents Alerts!
Please note: CJC is published in English and Chinese. Manuscripts are published online prior to printing. The citation will change as volume, issue and page numbers are finalized.
Email this page
Print this page
Basic Research Paper
Overexpressing Exogenous S100A13 Gene and Its Effect on Proliferation of Human Thyroid Cancer Cell Line TT
Ren-Xian Cao, Li-Na Tian, Fang Wen, Xing Liu, Jing Zhong and Ge-Bo Wen
volume 27 | issue 8
August 2008Subscribe to this journal for $0/year
Background and Objective: S100A13 is involved in tumor formation, and is highly expressed in human thyroid gland. This study was to investigate the effect of exogenous S100A13 overexpression on the proliferation of human thyroid cancer cell line TT. Methods: The eukaryotic expression plasmid pCDNA3.1/NT-GFP-S100A13 and empty vector pCDNA3.1/NT-GFP were transfected into TT cells. The cells were selected by G418. The expression of green fluorescent protein (GFP) was observed under laser scanning microscope, and the expression of S100A13 mRNA and protein was detected by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The effects of S100A13 on cell proliferation and cell cycle progression were measured by cell growth curve and flow cytometry. Results: TT-S100A13-GFP and TT-GFP cells, which separately expressed S100A13 and pCDNA3.1/NT-GFP, were constructed successfully. TT-S100A13-GFP cells grew faster than TT-GFP and TT cells [(2.30 ± 0.24) × 105 vs. (1.40 ± 0.25) × 105 and (1.50 ± 0.22) × 105 at the seventh day of cell culture, p < 0.05]; both S phase proportion and G2/M phase proportion were significantly higher in TT-S100A13-GFP cells than in TT-GFP and TT cells [(6.47 ± 0.14)% vs. (5.86 ± 0.23)% and (5.99 ± 0.28)% at S phase, p < 0.05; (50.27 ± 0.66)% vs. (39.39 ± 0.23)% and (39.64 ± 0.64)% at G2/M phase, p < 0.05]. Conclusion: Exogenous S100A13 gene overexpression could accelerate cell proliferation, and promote cell cycle progression of TT cells from G0/G1 phase to S and G2/M phase.
Authors
Ren-Xian Cao
University of South China
Li-Na Tian
University of South China
Fang Wen
University of South China
Xing Liu
University of South China
Jing Zhong
University of South China
Ge-Bo Wen
University of South China