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Brief Report

Coordination of mitochondrial bioenergetics with G₁ phase cell cycle progression

Stefan M. Schieke, J. Philip McCoy Jr. and Toren Finkel

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Relatively little is known regarding how energetic demand during cell proliferation is sensed or coordinated with mitochondrial metabolism. Here we demonstrate that cell cycle progression through G1 is associated with a significant increase in mitochondrial membrane potential (∆Ψm) and respiration. We used this change in metabolic rate to isolate cells in G1 with low and high levels of mitochondrial membrane potential (∆ΨmL and ∆ΨmH). Biochemical and functional studies demonstrate that ∆ΨmL and ∆ΨmH cells display the distinct characteristics of early and late G1 phase, respectively. We further demonstrate that the metabolic rate in G1 reflect levels of the mTOR-raptor complex as well as susceptibility to rapamycin-induced cell cycle delay. In conclusion, our data suggests a coupling of mitochondrial bioenergetics and G1 progression and points to the mTOR signaling pathway as a potential molecular coordinator of these two processes.

Authors

Stefan M. Schieke

National Institutes of Health; Bethesda, MD

J. Philip McCoy Jr.

National Institutes of Health; Bethesda, MD

Toren Finkel

National Institutes of Health; Bethesda, MD

Purchase article for $19

Subscribe to this journal for $129/year