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Reports

p14^ARF is capable of promoting HIV-1 Tat degradation

Barbara Gargano, Marianna Fiorillo, Stefano Amente, Barbara Majello and Luigi Lania

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The p14^ARF tumor suppressor functions as ʻoncogenic checkpoint' that prevents unrestricted cellular proliferation in response to oncogenic signaling. Albeit, the major pathway through which ARF operates is the ARF-Mdm2-p53 axis, ARF directly binds to and inactivates transcription function of a number of DNA-bound activators. In the present study we show that p14ARF inhibits transcription activation of HIV-1 LTR promoter activity by Tat protein. Tat protein is a RNA-bound transcriptional activator whose function is strictly required for HIV-1 replication. We determined that p14^ARF inhibits Tat transactivation of HIV-1 LTR by promoting Tat degradation via an ubiquitinindependent pathway.

Authors

Barbara Gargano

University of Naples 'Federico II'; Naples, Italy

Marianna Fiorillo

University of Naples 'Federico II'; Naples, Italy

Stefano Amente

Naples Oncogenomic Center (NOGEC); Naples, Italy

Barbara Majello

University of Naples 'Federico II'; Naples, Italy

Luigi Lania

University of Naples 'Federico II'; Naples, Italy

Purchase article for $19

Subscribe to this journal for $129/year