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Perspectives

Molecular imaging of protein kinases

Limin Zhang, Mahaveer S. Bhojani, Brian D. Ross and Alnawaz Rehemtulla

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Protein kinases are important regulators of signal-transduction pathways. Dysregulated kinase activity is observed in a variety of human diseases such as cancer, making them targets for the development of molecular therapies. Identification of new drugs is greatly aided by molecular imaging tools which enable real time, non-invasive, dynamic and quantitative imaging of kinase activity in vivo. We have recently described a new reporter platform based on conformation dependent complementation of firefly luciferase to monitor serine/threonine kinase (Akt) activity. The reporter system provides unique insights into the pharmacokinetics and pharmacodynamics of drugs that modulate kinase activity in living subjects and also provide a platform for cell based high-throughput drug screening for modulators of kinase activity.

Authors

Limin Zhang

University of Michigan Medical School; Ann Arbor, MI

Mahaveer S. Bhojani

University of Michigan Medical School; Ann Arbor, MI

Brian D. Ross

University of Michigan Medical School; Ann Arbor, MI

Alnawaz Rehemtulla

University of Michigan Medical School; Ann Arbor, MI

Purchase article for $19

Subscribe to this journal for $129/year