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A Transgenic Mouse Model for High Content, Cell Cycle Phenotype Screening in Live Primary Cells

Richard O. Burney, Alan I. Lee, Denise E. Leong, Joshua T. Jones, Angela T. Hahn, Tobias Meyer and Mylene W.M. Yao

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High content cell-based genetic and small molecule library screens are powerful strategies in drug discovery and investigations of disease mechanisms. We report that primary cells derived from a transgenic mouse model expressing a fluorescence mitosis biosensor provide unambiguous phenotype readouts without the need for transfection or immunocytochemistry. Phenotype profiles of cell cycle disruption and of apoptosis are easily detectable at a single time point selected from time-lapse live fluorescence microscopy. Most importantly, this transgenic mouse model may be crossed with cancer mouse models to derive biosensor-expressing primary cancer cells for use in high content screening strategies targeting discovery of tumor-specific chemotherapeutic compounds.

Authors

Richard O. Burney

Stanford University; Stanford, California

Alan I. Lee

Stanford University; Stanford, California

Denise E. Leong

Stanford University; Stanford, California

Joshua T. Jones

Stanford University; Stanford, California

Angela T. Hahn

Stanford University; Stanford, California

Tobias Meyer

Stanford University; Stanford, California

Mylene W.M. Yao

Stanford University; Stanford, California

This is an open-access article

 Download PDF

If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.