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ORC Function in Late G1: Maintaining the License for DNA Replication
Lance Da-Silva and Bernard P. Duncker
volume 6 | issue 2
15 January 2007Pages: 128 - 130
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The origin recognition complex (ORC) is essential as a scaffold for the assembly of pre-replicative complexes (pre-RCs) in G1 phase of the cell cycle. Some models have proposed that once origins have been licensed for DNA replication, ORC is dispensable for MCM protein association, and ensuing DNA replication. Although budding yeast Orc6 is not needed for origin recognition or binding in vitro, we have recently shown that this ORC subunit is required in late G1 phase for maintenance of MCMs, and subsequent DNA replication. Further investigation shows that depletion of Orc6 results in displacement of MCM proteins from both early- and late-firing origins, and eventually results in the activation of the Rad53 checkpoint kinase, consistent with incomplete DNA replication. Loss of MCM association at origins may be mediated by the displacement of Mcm10 and/or Orc2 as a consequence of late G1 Orc6 depletion.
Authors
Lance Da-Silva
University of Waterloo; Biology; W. Waterloo, Ontario, Canada
Bernard P. Duncker
University of Waterloo; Biology; W. Waterloo, Ontario, Canada
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.