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Brief Report
Ectopic Expression of Plk1 Leads to Activation of the Spindle Checkpoint
Jiabin Tang, Raymond L. Erikson and Xiaoqi Liu
volume 5 | issue 21
1 november 2006Pages: 2484 - 2488
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Polo-like kinase 1 (Plk1), the best characterized member of the mammalian polo-like kinase family, is well regulated throughout the cell cycle, and is inhibited following DNA damage. Chk1 plays a key role in the response to DNA damage. We recently reported that Chk1 is required for mitotic progression through negative regulation of Plk1. Here, we report the phenotypes of cultured cells upon ectopic expression of various forms of Plk1. Epitopic expression of Plk1 led to mitotic arrest, whereas co-expression of Chk1 could release this mitotic block. Moreover, the Plk1 expression-induced mitotic block was also released by inactivation of the spindle-assembly checkpoint.
Authors
Jiabin Tang
Purdue University, West Lafayette, IN
Raymond L. Erikson
Harvard University, Cambridge, MA
Xiaoqi Liu
Purdue University, West Lafayette, IN
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.