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The Second Subunit of the Replication Factor C complex (RFC40) and the Regulatory Subunit (RI?) of Protein Kinase A form a Protein Complex Promoting Cell Survival
Rakhee S. Gupte, Yun Weng, Li Liu and Marietta Y.W.T. Lee
volume 4 | issue 2
february 2005Pages: 323 - 329
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Replication Factor C (RFC) is required for the loading of Proliferating Cell Nuclear Antigen (PCNA) onto DNA during DNA replication, repair and recombination. RFC40, the second subunit of the RFC complex, and PCNA have been shown to be over-expressed in gestational trophoblastic diseases. Using RFC40 as the bait in a yeast two-hybrid screening, we have identified a novel interaction between RFC40 and the regulatory subunit (RI?) of cAMP-dependent Protein kinase A (PKA). The interaction sites between these two proteins were investigated and mapped to the N-terminus of RI? and the C-terminus of RFC40. Moreover, it was demonstrated that the C-subunit of PKA was not associated with the RFC40-RI? complex. Furthermore, RFC37, the third subunit of the RFC complex, competes with RI? and displaces it from the RFC40-RI? complex. Interestingly, down regulation of endogenous RI? by 8-chloro cAMP, in MCF7 breast cancer cells led to reduction in the amount of RFC40-RI? complex, together with decrease in cell survival.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.