Sign up for Table of Contents Alerts.
Email this page
Print this page
Research Paper
p21 (WAF1/CIP1) Mediates the Growth Response to TGF-b in Human Epithelial Cells
Kurtis E. Bachman, Brian G. Blair, Keith Brenner, Alberto Bardelli, Sabrina Arena, Shibin Zhou, Jessica Hicks, Angelo De Marzo, Pedram Argani and Ben Ho Park
volume 3 | issue 2
feb 2004Pages: 221-225
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.
We investigated the mechanism by which cancers evade the growth inhibitory effects of TGF-b. Using two p21-/- somatically deleted human epithelial cell lines, we find that TGF-b serves as a growth stimulator rather than a growth suppressor to cells lacking p21. In addition, TGF-b stimulated p21-/- cells exhibited a mesenchymal phenotype, demonstrated by an upregulation of vimentin and decreased expression of E-cadherin. Analysis of primary human breast cancers by immunohistochemical labeling confirmed a correlation between p21 loss and positive vimentin expression. These data provide a molecular mechanism explaining how non-gastrointestinal cancers can escape the anti-proliferative effects of this cytokine and simultaneously use this pathway for growth advantage.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.