Sign up for Table of Contents Alerts.
Email this page
Print this page
Research Paper
JSI-124 inhibits glioblastoma multiforme cell proliferation through G2/M cell cycle arrest and apoptosis augment
Yuhang Su, Gang Li, Xulong Zhang, Jinhai Gu, Cai Zhang, Zhigang Tian and Jian Zhang
volume 7 | issue 8
August 2008Subscribe to this journal for $129/year
JSI-124(cucurbitacin I) is a selective inhibitor of Janus kinase /Signal transducer and activator of transcription 3(JAK/STAT3) and has been shown to exert anti-proliferative and anti-tumor properties both in vitro and in vivo. As STAT3 activation has been implicated in the development of glioma, we investigated the therapeutic efficacy of JSI-124 on glioblastoma multiforme (GBM) by interfering with STAT3 pathway. In present study, two GBM cell lines, U251 and A172 cells, were treated with JSI-124. The results showed that the cell growth was inhibited significantly in a dose-and time-dependent manner. Further investigation illustrated that the levels of phosphorylated-STAT3 were decreased in GBM cells treated by JSI-124, concomitant with apoptosis augment and cell cycle arrest. Specially, JSI-124 induced G2/M accumulation via down-regulation of cyclin B1 and cdc2 expression. Together these results suggested that inhibition of STAT3 by JSI-124 is a potential strategy for the development of the new glioblastoma multiforme theraputics.