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Research Paper
CD95 Rapidly Clusters in Cells of Diverse Origins
Jessica C. Fanzo, Maureen P. Lynch, Hyewon Phee, Marc Hyer, Aida Cremesti, Heike Grassmé, James S. Norris, K. Mark Coggeshall, Bo R. Rueda, Alessandra B. Pernis, Richard Kolesnick and Erich Gulbins
volume 2 | issue 4
july/august 2003Pages: 392-395
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We have shown that CD95-mediated cell death requires a clustering of the receptor in distinct sphingolipid-rich domains of the cell membrane (Grassmé et al., 2000, Cremesti et al., 2000). These domains form in response to acid sphingomyelinase (ASM)-induced ceramide generation. However, recent studies challenged the finding of early CD95 clustering (Algeciras-Schimnich et al., 2002). Here, six independent groups tested clustering of CD95 in diverse cell type including primary cells ex vivo and established cell lines. The studies show clustering of CD95 within seconds to minutes in all cell types tested by the different groups. In addition, clustering of CD95 was detected after stimulation of cells using three agonistic anti-CD95 antibodies (CH11, APO-1-3 and JO2), CD95 ligand and stimuli that induce an upregulation and activation of the endogenous CD95/CD95 ligand system. The data confirm our previous studies and suggest rapid, i.e. within seconds to minutes, CD95 clustering as a general phenomenon occurring in many cell types.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.