Sign up for Table of Contents Alerts.
Email this page
Print this page
Research Paper
Evidence for the Involvement of Purα in Response to DNA Replication Stress
Huichen Wang, Meijuan Wang, Krzysztof Reiss, Nune Darbinian-Sarkissian, Edward M. Johnson, George Iliakis, Shohreh Amini, Kamel Khalili and Jay Rappaport
volume 6 | issue 4
April 2007Pages: 596 - 602
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.
Purα is a sequence-specific nucleic acid binding protein that is involved in multiple cellular functions including regulation of transcription, initiation of DNA replication, cell cycle progression, and neuronal cell differentiation. Its potential role in DNA repair has not been explored. We have now analyzed the role of Purα in the cellular response to replication-associated DNA repair of double-strand breaks (DSBs) using Purα knockout mouse embryo fibroblasts (MEFs). We found that Purα negative cells are hypersensitive to the DNA replication inhibitor, hydroxyurea (HU), and that HU induces excessive DSBs, which delayed the resumption of cell cycle progression after HU treatment. Reintroduction of Purα into Purα null cells reduced the accumulation of DSBs and enhanced DNA end joining. These results suggest a role for Purα as a caretaker protein that is involved in the repair of DSBs induced by stalled replication forks.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.