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Brief Communication
Gefitinib-Sensitizing Mutation in Esophageal Carcinoma Cell Line Kyse450
Mingzhou Guo, Shuang Liu, James G. Herman, Hui Zhuang and Fengmin Lu
volume 5 | issue 2
february 2006Pages: 152-155
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Purpose: The sensitivity of lung cancer to gefitinib has been found to be associated with mutations at the tyrosine kinase domain of epidermal growth factor receptor (EGFR), yet similar observations are not available in other solid tumors. We recently identified mutations in the EGFR kinase domain in primary esophageal carcinoma. The purpose of this study was to investigate if they are gefitinib-sensitizing EGFR mutations.
Experimental Design: The gefitinib sensitivity of human esophageal tumor cell line Kyse450 , which harboring a EGFRS7681 mutation, and TE8 without EGFR mutation were analyzed, and a human non-small cell lung cancer cell line H358 reported insensitive to gefitinib was used as a control. The effect of EGFRS7681 mutation on cell growth and apoptosis was assessed.
Results: As demonstrated by in vitro proliferation assay, this mutation sensitized Kyse450 cells to gefitinib. The observation of down regulation of the phosphorylated pAKT indicated gefitinib induced Kyse450 cells apoptosis via inhibition of EGFR activity.
Conclusions: While more primary esophageal tumors remain to be screened for the mutations at the tyrosine kinase domain of EGFR, current observation implies that gefitinib may be worth further investigation for treatment of esophageal cancers.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.