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Bedside to Bench
Paraneoplastic Leukemoid Reaction and Rapid Progression in a Patient with Malignant Melanoma: Establishment of KT293, a Novel G-CSF-Secreting Melanoma Cell Line
Bodo Schniewind, Matthias Christgen, Axel Hauschild, Roland Kurdow, Holger Kalthoff, Hans Jürgen Klomp
volume 4 | issue 1
january 2005Pages: 023-027
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Paraneoplastic leukemoid reaction (PLR) is a rare condition of leucocytosis in cancer patients. Here we report the rapid progression of a patient suffering from a metastasized malignant melanoma and PLR. The patient’s white blood cell count exceeded 200,000 cells per µl and the serum level of Granulocyte Colony-Stimulating Factor (G-CSF) was elevated up to 780 pg/µl. A Tc-m99-labeled anti-NCA90/95 based granulocyte scan demonstrated reactive bone marrow expansion, splenomegaly and granulocyte infiltration into the tumor. KT293, a S100, gp100 and CD68 positive melanoma cell line derived from an axillary metastasis, produced large amounts of G-CSF in vitro and induced rapidly growing tumors and PLR after subcutaneous inoculation in SCID mice. In contrast to G-CSF-secreting cancer cells of other tissue origin, G-CSF-neutralizing antibodies failed to inhibit the growth of KT293 cells. In addition, KT293 cells did not express G-CSF-receptor. These observations suggest that paracrine effects of G-CSF-secretion and PLR might promote an aggressive melanoma phenotype, as seen in this patient.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.