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Research Paper
Alterations of p53 and Bcl-2 Protein Expression in the Laryngeal Intraepithelial Neoplasia
Mahmoud R. Hussein
volume 4 | issue 2
February 2005Pages: 213-217
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BACKGROUND: The squamous cell carcinoma of the larynx (SCC) is a common head and neck malignancy. I hypothesize that the development of laryngeal intraepithelial neoplasia (LIN) involves alterations of tumor suppressor genes (p53) and protooncogenes (Bcl-2). METHODS: To test this hypothesis, 40 vocal cord biopsy specimens were examined for p53 and Bcl-2 protein expression using immunoperoxidase staining methods and mouse monoclonal antibodies. RESULTS: The lesions appeared as localized , flat or papillary areas with white (leukoplakic) red (erythroplakic), or gray appearance. Histologically, the lesions entailed variable associations of mild (LIN I), moderate (LIN II), sever dysplasia (LIN III or in situ carcinoma) and superficially invasive SCC. As compared to the normal mucosa, the average weighted immunoreactivity scores for p53 and Bcl-2 proteins showed significant upregulation with the transitions from LIN I to LIN II to LIN III to superficially invasive SCC , respectively (0.0± 0.0, 0.0± 0.0, 1.8 ± 0.8, 2.3 ± 0.8, 5.8 ± 3.2, p= 0.00 for p53 ) and (4.4 ± 0.4, 4.0 ± 0.8, 6.0 ± 2.0, 9.6 ± 1.6 and 10.7± 1.3, p= 0.00 for Bcl-2). There were insignificant positive correlation between p53 and both Bcl-2 (r=+0.8, p>0.13) protein expression. CONCLUSIONS: Alterations of the p53 and Bcl-2 proteins occur during the development of SCC.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.