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Research Paper

Proteomic Analysis of Membrane-Associated Proteins from Rat Liver Autophagosomes

Anders Øverbye, Monica Fengsrud and Per O. Seglen

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Proteins associated with membranes from purified rat liver autophagosomes were separated by two-dimensional (2D) gel electrophoresis (zoom gels, pI 4-7 and 6-9), silver-stained and identified by MALDI-TOF mass spectrometry. Among >1,500 detectable protein spots, 58 (derived from 39 different known proteins) were at least twofold (and significantly) enriched in autophagosomal membranes relative to cytoplasmic membranes. All of these membrane-associated proteins were also present in the cytosol, many of them being truncated enzyme variants that would be expected to serve a binding rather than an enzymatic function.

Authors

Anders Øverbye

University of Oslo

Monica Fengsrud

University of Oslo

Per O. Seglen

Department of Cell Biology; Institute for Cancer Research; Rikshospitalet HF and Department of Molecular Biosciences; University of Oslo; Oslo, Norway

This is an open-access article

 Download PDF

If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.