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Review

Methods for Monitoring Autophagy from Yeast to Human

Daniel J. Klionsky, Ana Maria Cuervo and Per O. Seglen

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The increasing interest in autophagy in a wide range of organisms, accompanied by an ever-growing influx of researchers into this field, necessitates a good understanding of the methodologies available to monitor this process. In this review we discuss current approaches that can be used to follow the overall process of autophagy, as well as individual steps, from yeast to human. The majority of the review considers methods that apply to macroautophagy; however, we also consider alternative types of degradation including chaperone-mediated autophagy and microautophagy. This information is meant to provide a resource for newcomers as well as a stimulus for experienced researchers who may be prompted to develop additional assays to examine autophagy-related pathways.

Authors

Daniel J. Klionsky

Life Sciences Institute and Department of Molecular, Cellular and Developmental Biology, and Department of Biological Chemistry; University of Michigan; Ann Arbor, Michigan USA

Ana Maria Cuervo

Albert Einstein College of Medicine

Per O. Seglen

Department of Cell Biology; Institute for Cancer Research; Rikshospitalet HF and Department of Molecular Biosciences; University of Oslo; Oslo, Norway

This is an open-access article

 Download PDF

If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.